Quantitation of 14C-Oxaliplatin Concentrations in Human Serum Samples by Using Accelerator Mass Spectrometry

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著者:Takeshi KOBAYASHI, Teiko TOYOGUCHI, Kazuhiro KATO, Fuyuki TOKANAI, Tadashi SHIRAISHI

抄録:The understanding of human pharmacokinetics is important for development of new drugs. Microdosing studies have been proposed as means of obtaining human pharmacokinetics information at early stages of drug development. Accelerator mass spectrometry (AMS) has high detection sensitivity and is expected to play an important role in microdose trials. In this study, we used the AMS microdosing facility at Yamagata University to measure the concentration of 14C in 14C-oxaliplatin-spiked serum samples. The calibration curve of 14C concentration in serum was linear, and the correlation coefficient was 0.9994. The precision, accuracy, and stability values obtained (freeze and thaw cycles, and short- and long-term stability) satisfied the criteria. The mean background 14C concentrations in samples of 6 healthy Japanese volunteers were 1.635dpm/mL in blood and 0.56dpm/mL in plasma. These results suggest the suitability of AMS-based quantitation for analyzing samples from microdosing studies.